JEOL Cryo-Tomography Workshop

From sample to structure — instruments and workflows for cryo-electron tomography

Satellite workshop preceding the 21st International Microscopy Congress.

Sunday 30 August   ·   10:30 – 17:00   ·   University of Liverpool

Register HERE.

Time       Programme
10:30 Registration and Coffee
11:00   Introduction 
11:05  Naoki Hosogi
Getting Started with Cryo-ET: An Overview of the Instruments and the Workflow
11:25  Q & A
11:30  Prof. David Bhella
Fundamentals of Cryo-ET
11:55  Q & A
12:00 Lunch
13:00 James Streetly
Setting up batch cryo-ET data collection with SerialEM
13:25  Q & A
13:30  Fabian Eisenstein
High-throughput Tilt Series Acquisition with PACEtomo and SPACEtomo
13:55  Q & A
14:00  Prof. Koji Yonekura
Multimodal cryo-EM approaches towards cryo-ET 
14:25 Q & A
14:30  Fumiaki Makino
From Data Collection to Structure: A Subtomogram Averaging Workflow Using JEOL Cryo-EM
14:55  Q & A
15:00 Break
15:30  Prof. Roland Fleck
Cryo-FIB-SEM for Structural Biology — Streamlining the Workflow
15:55  Q & A
16:00  Stephen Carter
Cryo-ET captures the translational machinery in senescent cells
16:25 Q & A
16:30 Discussion Time